In each cross, seeds from each of the 4 generations
were tested against the insects by putting
into each plastic box 50 seeds from individual F1
plant, or 20 seeds from individual F2 or individual
BC1F1 plant. The bruchids C. chinensis and C.
maculatus were reared on susceptible mungbean
seeds and 1–3 day old adults were used to infest
the seeds. Forty beetles were released in each
box, kept for 7 days, and then removed. Since the
difference in seed size and seed coat may affect
the egg laying of the bruchids, in concurrent to
insect removal the seeds were checked to ensure
that every of them had eggs. Parental seeds were
also assessed as controls. The test condition was
performed at 28–30C, 65–70% RH. Number of
damaged seeds was counted and recorded at 35
and 50 days after insect introduction. The damaged
seeds were removed from the boxes after
counting. The total number of damaged seeds
from each plant was converted into percentage.
In each cross, seeds from each of the 4 generationswere tested against the insects by puttinginto each plastic box 50 seeds from individual F1plant, or 20 seeds from individual F2 or individualBC1F1 plant. The bruchids C. chinensis and C.maculatus were reared on susceptible mungbeanseeds and 1–3 day old adults were used to infestthe seeds. Forty beetles were released in eachbox, kept for 7 days, and then removed. Since thedifference in seed size and seed coat may affectthe egg laying of the bruchids, in concurrent toinsect removal the seeds were checked to ensurethat every of them had eggs. Parental seeds werealso assessed as controls. The test condition wasperformed at 28–30C, 65–70% RH. Number ofdamaged seeds was counted and recorded at 35and 50 days after insect introduction. The damagedseeds were removed from the boxes aftercounting. The total number of damaged seedsfrom each plant was converted into percentage.
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