After being removed from the centrifuge, we transferred 40 micro liters of the supernatant extract to test tubes, being added more 460 micro liters of distilled water and 1.0 mL of the reagent Bio-Rad.Thesolutions were stirred and, conditioned in cuvettesin order to conduct the reading at 630 nanometers in spectrophotometer, obtaining, thus, the value of absorbance.