Each umami amino acid (aspartic [Asp] and
glutamic acids [Glu]) and 50-nucleotide were identified using the
authentic amino acid and 50-nucleotide , respectively and then quantified by the calibration
curve of the authentic compound. EUC [g monosodium glutamate
(MSG) per 100 g] is the concentration of MSG equivalent to
the umami intensity given by the mixture of MSG and the 50-nucleotide
and is represented by the following addition equation