Objective: To investigate calcium and phosphorus levels in the bones of low value fishes Sardinella
longiceps (oil sardine) and Trichiurus savala (ribbon fish). Methods: Bones and skeletal remains
were subjected to alkaline-alcohol treatment for specified period of time and were then dried and
pulverized. Calcium and phosphorus levels were determined by both volumetric and instrumental
methods using atomic absorption spectroscopy. Results: Volumetric analysis of calcium and
phosphorus were 28.98% and 14.2% in oil sardine; 24.2% and 11.6% in ribbon fish respectively. Atomic
absorption spectroscopy analysis of calcium and phosphorus were 32.73% and 17.2% for oil sardine
and 27.17% and 10.83% for ribbon fish respectively. Protein level was 4.82% in oil sardine and 3.97% in
ribbon fishes. Conclusions: The findings of the present study revealed that sardine bones are rich in
calcium and will be utilized for the production of calcium powder to treat osteoporosis.