At the completion
of the culture cycle, cell density was again measured by hemocytometer
counting, and subsamples were prepared for dry weight analysis. Aliquots of 50 ml culture were harvested by centrifugation at 4800 × g for 20 min; the supernatant containing spent media soluble solids and pellet consisting of cells and insoluble papaya solids were separately dried in foil weigh boats at 105 °C overnight prior to weighing.