In this research study Species diversity of mushroom in Thung Khai Botanical garden , Trang Province was conducted during June - August 2015 and November 2015 – January 2016. Consised of two route , first , bike route for 5 Kilometer , Secound , nature trail for 3 Kilometer , Survey and collected for study and identified by their scientific names and generic level. This was achieved comparing speciemens with descriptions and photographs in the references and keys. In this , 6 order 16 family 27 species were found. Saparated for poison and non poison mushroom were found 12 species and 15 species expecfively.