Sample Collection and Preparation: Fresh plants of Ulva lactuca linn, Sargassum wightii Greville, Padina gymnospora (Kutz) Vicker and Gracilaria edulis (Gmelin) Silva, were collected from the intertidal region of the Pudumadam (Lat 9°15' N; Long 79° E) southeast coast of India. Then the plants were washed thoroughly with sea water to remove extraneous materials and brought to the laboratory in plastic bags containing water to prevent evaporation. Samples were then shade dried in
till constant weight obtained and ground in an electric mixer. The powdered samples subsequently stored in refrigerator.