A completely randomized design (CRD) was adopted as an experimental design for bacterial isolation. Samples were collected from each of the three composts and the methodology used for bacterial isolation was serial dilution and plating onto nutrient agar plates [18]. For each dilution, three replicates were maintained. The plates were incubated at 37 °C for 24 h and results were observed.
The plates were observed and counted the next day; morphologically different and distinct bacterial colonies were selected and subcultured to new nutrient agar slants for further screening.