Residue was dissolved in
5 ml of 0.4% sodium hydroxide solution, followed by addition of
3 ml of 3% phosphomolybdic acid and allowed to stand for 1 h.
The solution was filtered and centrifuged at 5000 rpm for 10–
15 min. The absorbance of the blue colored solution was read at
650 nm against blank in a UV spectrophotometer. The concentration of capsaicin in the sample was calculated from the slope of
the standard curve and expressed as percent.