There was no significant difference in the moisture content and dry matter between cotton seeds fermented by inoculating with single starter cultures of B. subtilis and B. licheniformis. However, there was a great increase in the moisture content of fermented cotton seeds. The increase in the moisture content ranged from 39.26% to 55.85%% in the unfermented cotton seeds and those fermented when the combinations of all the four bacteria were used as starters, respectively. The increase in moisture content observed in the fermented seeds is due to the moist solid nature of the fermentation and the hydrolytic decomposition of the fermenting substrate. Also, there was an observed decrease in the dry matter of the fermented cotton seeds.