Selected microalgal strains were pre-cultured in 100 mL sterilized bottle reactor and incubated in 300 C with light intensity of 30 Watt/m2 in alteration of 12 h dark and 12 h light regime. After 5–7 days, when the cell density reaches 0.5 of absorbance (680 nm), sample was transferred to the 1 L culture vessel. This culture would be the stock for scale up cultivation to 5 L that was transferred gradually. The procedures were similar to the all isolates with their appropriate selected media. For running the experiment, the isolates were cultivated in cylindrical vessel reactor with 5 L working volume during 30 days in triplicate. Observed parameters are growth rate, proximate biomass and environmental condition such as temperature, pH and light intensity at per three days. The growth rate of each strain was determined by cell turbidity that measured by spectrophotometry (Shimadzu UV-1700) at 680 nm, comparing to medium as blank control [14].The environment factors were measured by portable kit for pH, temperature and light intensity.