cyclic voltammetry was used to evaluate H2O2 detection in a scan rate 10 mV/s within the voltage window of −0.1 to +0.4 V versus the Ag/AgCl reference electrode.
Amperometric tests were carried out with 0.1 M pH 7.5 phosphate buffer solutions with a 150 mM KCl as a supporting electrolyte at the H2O2 concentrations from 0 to 0.4 mmol L−1. An operation time of 60 s, at which the minimum steady-state response current was reached, was chosen to record the oxidation current of the H2O2. The reproducibility of the measurements was evaluated using a new biosensor prototype and each H2O2 concentration for 3 to 5 times. Figure 2 shows the experimental results, and the linear relationship between the current outputs of the biosensor prototype and the H2O2 concentrations exists.