Assay of Amylase:The amylase activity was determined following the method of Bernfeld [5]. An assay mixture enzyme extract, starch as substrate and DNS as coupling reagent was used. One unit of α - amylase activity was defined as the number of µ moles of maltose liberated by 1 mL of enzyme solution per minute.
RESULTS AND DISCUSSION
Amylase Production in Submerged Fermentation
In submerged fermentation the production of amylase was reached maximum of 4 U/ml at 10 h of incubation period (Fig. 1). Further increase in incubation period did not show any significant increase in enzyme production rather it was decreased. Thus optimum time of enzyme synthesis was to be 10 h after inoculation.