4. Conclusion
All the data obtained from surface analysis by CAM, AFM,
PM-IRRAS and XPS suggest that biosurfactants from L. helveticus
1181 and P. fluorescens 495 strains modify the stainless
steel surfaces in different ways: Pf495 biosurfactant conditioning
results in a thin homogeneous layer of organic material and
Lb conditioning leaves a rather smaller amount of adsorbed
molecules, in a more heterogeneous layer. Both biosurfactants
induce a net segregation of chromium towards the surface. In
our experimental conditions, this bioconditioning of stainless
steel surfaces led to a considerable decrease of the biocontamination
by strains of L. monocytogenes, averagely hydrophobic or
hydrophilic associated with a more or less strong electron donor
character. These results could be attributed to modifications in
the acid base characteristic of solid surfaces [5].
As a consequence, in our experimental conditions, we have
demonstrated the efficiency of conditioning a stainless steel surface
by a layer of biosurfactants, notably that produced by L. helveticus,
a strain of technological interest, on the adhesion of four
strains of L. monocytogenes presenting very different physicochemical
phenotypes. It is noticeable that this anti-adhesive
effect was observed by using biosurfactants released as well
by gram-negative bacteria (Pseudomonas) as by gram-positive
bacteria (Lactobacillus). This work shows the real antiadhesive
power of these biological surface-active compounds against biocontamination
of metallic surfaces by undesirable germs like L.
monocytogenes. The bioconditioning of solid surfaces, leading
to a significant decrease in listerial contamination, makes obvious
4. ConclusionAll the data obtained from surface analysis by CAM, AFM,PM-IRRAS and XPS suggest that biosurfactants from L. helveticus1181 and P. fluorescens 495 strains modify the stainlesssteel surfaces in different ways: Pf495 biosurfactant conditioningresults in a thin homogeneous layer of organic material andLb conditioning leaves a rather smaller amount of adsorbedmolecules, in a more heterogeneous layer. Both biosurfactantsinduce a net segregation of chromium towards the surface. Inour experimental conditions, this bioconditioning of stainlesssteel surfaces led to a considerable decrease of the biocontaminationby strains of L. monocytogenes, averagely hydrophobic orhydrophilic associated with a more or less strong electron donorcharacter. These results could be attributed to modifications inthe acid base characteristic of solid surfaces [5].As a consequence, in our experimental conditions, we havedemonstrated the efficiency of conditioning a stainless steel surfaceby a layer of biosurfactants, notably that produced by L. helveticus,a strain of technological interest, on the adhesion of fourstrains of L. monocytogenes presenting very different physicochemicalphenotypes. It is noticeable that this anti-adhesiveeffect was observed by using biosurfactants released as wellby gram-negative bacteria (Pseudomonas) as by gram-positivebacteria (Lactobacillus). This work shows the real antiadhesivepower of these biological surface-active compounds against biocontaminationof metallic surfaces by undesirable germs like L.monocytogenes. The bioconditioning of solid surfaces, leadingto a significant decrease in listerial contamination, makes obvious
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4. Conclusion
All the data obtained from surface analysis by CAM, AFM,
PM-IRRAS and XPS suggest that biosurfactants from L. helveticus
1181 and P. fluorescens 495 strains modify the stainless
steel surfaces in different ways: Pf495 biosurfactant conditioning
results in a thin homogeneous layer of organic material and
Lb conditioning leaves a rather smaller amount of adsorbed
molecules, in a more heterogeneous layer. Both biosurfactants
induce a net segregation of chromium towards the surface. In
our experimental conditions, this bioconditioning of stainless
steel surfaces led to a considerable decrease of the biocontamination
by strains of L. monocytogenes, averagely hydrophobic or
hydrophilic associated with a more or less strong electron donor
character. These results could be attributed to modifications in
the acid base characteristic of solid surfaces [5].
As a consequence, in our experimental conditions, we have
demonstrated the efficiency of conditioning a stainless steel surface
by a layer of biosurfactants, notably that produced by L. helveticus,
a strain of technological interest, on the adhesion of four
strains of L. monocytogenes presenting very different physicochemical
phenotypes. It is noticeable that this anti-adhesive
effect was observed by using biosurfactants released as well
by gram-negative bacteria (Pseudomonas) as by gram-positive
bacteria (Lactobacillus). This work shows the real antiadhesive
power of these biological surface-active compounds against biocontamination
of metallic surfaces by undesirable germs like L.
monocytogenes. The bioconditioning of solid surfaces, leading
to a significant decrease in listerial contamination, makes obvious
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