Microbial fuel cells can be used to generate hydrogen for use as an alternative fuel. When used for hydrogen production, the MFC needs to be supplemented by an external power source to get over the energy barrier of turning all of the organic material into carbon dioxide and hydrogen gas [2]. The standard MFC is converted to hydrogen production by keeping both chambers anaerobic and supplementing the MFC with 0.25 volts of electricity [6]. Hydrogen bubbles form at the cathode and are collected for use as fuel source [7]. Although electricity is used instead of generated as in normal MFCs, this method of producing hydrogen is very efficient because more than 90% of the protons and electrons generated by the bacteria at the anode are turned into hydrogen gas [8]. Conventional production of hydrogen requires 10 times the amount of energy as an adapted MFC, making the MFC the most efficient and environmentally-friendly way to generate hydrogen for use as a fuel.