Under aseptic precautions, a nonfasting venous blood was drawn from neonates into procoagulant vacutainers and serum bilirubin was analyzed by Roche automated clinical chemical analyzer by colorimetric assay. Urine sample was collected within one hour of initiation of phototherapy [10], in plastic neonatal urine collection bags (Minicom), which were shielded from light during collection by diapering the infant. The collected urine samples were kept in containers wrapped with aluminum foil and were frozen [10] till they were analyzed by UV excitable fluorescence method [15]. The baseline data and estimated biochemical values were documented and preserved for data analysis.