Biocidal activity against sulfate reducing bacteria. The
inhibition activity of the prepared compounds against the Sulfate
Reducing Bacteria (SRB) growth was measured using the serial
dilution method according to ASTM D4412-84 [14].
SRB-contaminated water was supplied from Qarun Petroleum
Co. (West Desert, Egypt). This water was used for microbial
inhibition test. The test has been subject to growth of about 1015
bacteria cell/ml. The prepared compounds were tested as biocide
for the SRB by dose of (5 10 4, 1 10 4, 7 10 5 and5 10 5 M/l). The system was incubated to contact time of 3.0 h; each system was cultured in SRB specific media for 21dayes
at 37–40 8C.Scheme