Following the above treatment of the samples four volumes of 80 % (/v/v) ethanol (preheated at 60 °C) were added
to precipitate total dietary fiber (TDF) as well as to remove depolymerized protein and glucose. The precipitation was
allowed to occur at room temperature for 60 min, followed by centrifugation (4000 rpm for 15 min), and washing of the
residual mass twice with 78 % and 95 % (volume/volume) of ethanol respectively and then with acetone. The washed
residual mass was then dried overnight in a vacuum oven at 60 °C