After that, the incessant stream cultivation was applied with the
mixture of BTCW and the raw mentioned above. Herein, it must be
noticed that the ratio of the former to the later was increased gradually
and the former became the only feed on the 5th week. The
BAF reactors were both operated at 15–25 C. The hydraulic retention
time (HRT) was kept at about 2 h. During the acclimation,
NaHCO3 was also added into the influent in order to regulate the
pH value in the range of 6.0–7.0.