MATERIALS AND METHODS
1. Plant materials
The 10 Thai rejuvenating herbal plants were collected from certain sources and the parts of
plant used were identified by the domestic traditional medicinal expert and the medicinal expert at
the largest herbal plant materials in Bangkok. The plant Thai names were adapted to plant scientific
name with the aid of the reference book (วงศ์สถิตย์ ฉั่วกุล และคณะ, 2543) (Table 1). The plant
materials were sliced into pieces and dried in hot air oven at 70ºC. The dried materials were ground
into powder and submitted to extraction with 95% ethanol for 7 days in the dark place. The
supernatants were filtered through No.1 filter paper and subsequently evaporated in the rotary