Essential oils are volatile, natural, complex compounds witha strong odor that are made by aromatic plants as secondarymetabolites [17]. They have potential health benefits, medicinal properties, and strong antioxidant–antimicrobial (bactericidal,veridical, and fungicidal) properties. These properties make thempossible substitutes for synthetic antioxidant–antimicrobial agentsto achieve oxidative and microbial stability and improve mechan-ical properties, resulting in safer food products [11,19]. Severalstudies on the antimicrobial and antioxidant activity of essen-tial oils alone or incorporated into biodegradable films have beenpublished [20–25]. Zataria multiflora Boiss is a spice plant by thelocal name of Avishan-e-Shirazi in Iran, belonging to the Lamiaceaefamily, used as a spice in a variety of Iranian foods. The planthas known medicinal, antioxidant, and antimicrobial properties.Carvacrol and thymol are the main phenolic compounds of theessential oil constituents of this plant. The FDA has approved theessential oil of this plant as safe for use as a food additive, andcan be applied in antimicrobial food packaging. Considering theseproperties, the possible applications for Avishan-e-Shirazi essentialoil as a component in edible films has received special attention