2.3. Analysis of the toxin by HPLC-FLD
The extract used for the toxicity assay was passed
through a cartridge column (Sep-Pack C18, Waters) and an
ultrafiltration membrane (Ultrafree C3GC, Millipore), and
then the filtrate was applied for HPLC-FLD analysis. HPLCFLD
analysis was performed on a reversed phase column
(Develosil C8, Nomura Chemicals) by the method of
Oshima (1995a). All chemicals and solvents used were
HPLC or analytical grade.