hus, 4-week-old B6 mice were inoculated i.p. with rLm-gp33 or, as a control, with rLm-np118 (rLm expressing an LCMV nucleocapsid epitope np118-126) (29). Two weeks later, mice were infected with either RA59gfp/gp33 or, as a control, RA59-gfp. At day 5, the peak of virus replication in the CNS, mice were sacrificed and the brains, livers, and spleens were harvested.