From a collection of bacterial isolates from rhizosphere of plants growing in the local field five were selected which were antagonistic to the pathogen Tom5. These were identified as Pseudomonas aeruginosaT1, Pseudomonas sp.BH25, Pseudomonas sp.AM12, Pseudomonas sp.AM13 and Pseudomonas putidaR6. All the strains had properties of plant growth promoting rhizobacteria. These were assessed for their potential to biocontrol the manifestation of the pathogen on tomato by evaluating seedling emergence, vigour of the germinated seedlings and survivability of the seedlings following their transplantation. Among the strains Pseudomonas sp. BH25 was found to be promising to combat the pathogenic effect of R. solanacearumTom5 in bioassays. In the present study, the pathogen Ralstonia solanacearumTom5 caused only 40% seedling emergence as compared to 76% in the control, while combination of the antagonist BH25 with the pathogen Tom5 (Tom5:BH25 at 1:10) improved the percentage of the seedling emergence and the value (75%) was almost similar to that of the control. Combination of BH25 with the pathogen also improved the fresh weight, dry weight and vigour index [(mean root length + mean shoot length) x percentage of emergence of seedlings] of the seedlings as compared to those in the pathogen treated ones and their values were almost similar to those of the control. Vigour index of the seedlings was reduced from 935 in the control to 237 in the Tom5 treated ones and the value was restored to 878 by combining a 10-fold high concentration of BH25 with the pathogen Tom5 during inoculation. It was concluded that biocontrol organisms could be isolated from rhizosphere and applied to bacterial wilt infested field to combat the disease infestation.