Fig. 5. Relative expression of the IPNV-VP4 gene in the IPNV infected fish, and in the
vaccinated and challenged fish group 45 days post-challenge. The fish were vaccinated
with pcDNA-VP2 through the diet on 3 consecutive days and they were then challenged
with the virus (5 105 TCID50 mL1) after 30 days. A second group was mockvaccinated
and infected with IPNV (virus control group). Transcription of the VP4 gene
was recorded in the kidneys of both vaccinated and virus control fish at 45 days postchallenge,
and the data are represented relative to EF1-a expression (2DCt method),
(mean þ S.E., n ¼ 3). Values with asterisks are significantly different (P 0.05).