Study of B. subtilis SPB1 Lipopeptide Biosurfactant Production Using Low-cost Substrates
Considering the advantages of the solid-state fermentation, we selected three parameters affecting mostly B. subtilis SPB1 biosurfactant production yield, solid substrate ratio, moisture and inoculum size. Moreover, we investigated the optimal composition for B. subtilis SPB1 biosurfactant production of the mixed substrates. To predict the levels of the solid substrate ratio, moisture content and inoculum size, a central composite design was employed. After these experiments were performed, the production yields were determined ( Table 1). For the analysis of the results ( Table 1), a cubic response surface model was used. So, the following regression equation that reflects the empirical relationships between production yield under solid-state fermentation and the test variables in coded units was obtained.