Collection and preparation of cuttlefish skin Dorsal skin of cuttlefish (Sepia pharaonis) was obtained from a dock in Songkhla province. Cuttlefish skin was stored in ice with a skin/ice ratio of 1:2 (w/w) and transported to Department of Food Technology, Prince of Songkla University with in 1 h. Upon arrival, cuttlefish skin was washed with tap water and cut into small pieces (1×1 cm), placed in polyethylene bags and stored at −20°C until use. Storage time was not longer than 2 months. Prior to extraction, the frozen skin was thawed using running water until the core temperature reached 10°C