Materials and Methods
Propolis origins: propolis samples were collected from
different locations in Ardebile province in may 2005 and
pooled. Hand-collected propolis samples were kept in
the dark up to their processing and stored at -20 C o
Preparation of Ethanolic Extracts of Propolis (EEP)
Solution Propolis samples were cut into small pieces,
ground to a fine powder and extracted with 96% ethanol.
Let’s see how a ethanolic extract of propolis can be
made (Krell, 1996).
Take 30-40 grams of propolis powder and mix it with 70
ml of 90-96% alcohol. Leave for a minimum of 14 days,
in a dark cool place (not in refrigerator), but stir also 3-6
times per day, or find a mechanical way to do this
automatically. It is important to allow the alcohol
molecules to come into contact with as many propolis
compounds as possible, in order to extract them from
the solid mass.
After two weeks (The longer the better) the solution can
be filtered ; The liquid portion should be stored in a dark