B. variegata is the most studied Bauhinia
species. Mathur and Mukunthakumar (1992) used
nodal explants of mature trees to induce 7.8 shoots
per nodal segment in the presence of 13.3 μM BA
on MS basal medium containing 3% (w/v) sucrose
and 100 mg·dm-3
myo-inositol at a PPFD of
36 μmol·m
-2
·s
-1
and under a 16-h photoperiod at
28 °C. 96% of shoots rooted in the presence of
4.9 μM IBA. Thonnalak (2012) used axillary meristems
as explants to induce shoots on MS basal medium
containing 2.5 mg·dm-3 BA, although callus
formed when the BA concentration exceeded
5 mg·dm-3
. Shoots could be proliferated with 1-2.5
mg·dm-3 BA at 2500 Lux and a 16-h photoperiod
and formed roots when exposed to 5 mg·dm-3
NAA. Papafotiou et al. (2010) chose to use ex
vitro-derived shoot tips and single node explants
from young shoots (25 or 80 cm long) with four
apical nodes, which developed after pruning oneyear
old seedlings, as explants. The authors also
tested shoot tip or single node explants from 3-leaf
(two-week old) seedlings. Explants of the 3rd, 4th
and 5th node had a 70-100% response and 1.5-
2.0 cm shoots with 3-5 leaves represented the most