During semi conservative DNA replication, for example, the progressive unwinding of the DNA template requires a swivel in the DNA duplex to alleviate the over winding of the stands ahead of the moving replication fork. Of course, the replication apparatus may simple follow the helical path of the DNA template strands. However, this soon leads to a second problem of how to unlike the inter wound DNA helices following the completion of DNA synthesis. this concatenation of daughter molecules is absolutely required in the case of circular genomes and plasmids,in which the template strands are physically linked circles. similar considerations apply to the process of transcription, where the movement of a transcription complex along the DNA template may also produce a local unwinding of the DNA behind and over winding of the DNA ahead. This may be viewed as the formation of local domains of negatively and positively super-coiled DNA, respectively. Indeed, the translocation of any complex that forms between the two strands of a DNA duplex
for example
During semi conservative DNA replication, for example, the progressive unwinding of the DNA template requires a swivel in the DNA duplex to alleviate the over winding of the stands ahead of the moving replication fork. Of course, the replication apparatus may simple follow the helical path of the DNA template strands. However, this soon leads to a second problem of how to unlike the inter wound DNA helices following the completion of DNA synthesis. this concatenation of daughter molecules is absolutely required in the case of circular genomes and plasmids,in which the template strands are physically linked circles. similar considerations apply to the process of transcription, where the movement of a transcription complex along the DNA template may also produce a local unwinding of the DNA behind and over winding of the DNA ahead. This may be viewed as the formation of local domains of negatively and positively super-coiled DNA, respectively. Indeed, the translocation of any complex that forms between the two strands of a DNA duplexfor example
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