For preparation of the spraying solutions, good quality tap
water (EC: 0.25 mmhos cm1, pH: 7.14) was used; the control
plants were sprayed with tap water (0 mg Mn or Zn l1);
approximately 600 l of spray solution was applied per hectare;
a second spraying was carried out 4 weeks after the first application
using the above mentioned spraying method.