Overall, EL resulted in a rela tively high cis -9 18:1 co ncentration and an increase in the 18:3n -3/18:2n-6 ratio , wherea s combining EL and FO resulte d in subst a n tial increases in trans -FA, marginal enrichme nt in 20:5n -3 and 22:6n-3 and lower 16:0 conc entration ch anges associat ed with a decre ase in milk fat co ntent