Lactic acid is mostly produced by lactic acid bacteria
via fermentation of refined sugar [1]. Substrate cost is the main expense for lactic acid production. Starch
could be substituted for refined sugar to minimize the production cost. Nevertheless, this is still economically
unfavourable because pretreatment and enzymatic hydrolysis of starch is also costly [2]. A significant
reduction in these costs could be achieved by an application of amylolytic lactic acid bacteria (ALAB) for
simultaneous saccharification and fermentation (SSF) of starch to lactic acid [3]. In addition, among various
starches, cassava starch is the most attractive one in terms of availability and inexpensiveness [4]. National
Innovation Agency, Thailand, estimated that bioconversion of cassava root to polylactic acid could add value
to cassava root by 10 times [5]. However, the study on lactic acid production from SSF of cassava starch is
limited. Thus, the present research aimed at selecting an efficient amylolytic lactic acid bacterial strain and
determining some optimum conditions for SSF of cassava starch to lactic acid. The performance of the selected
strain in lactic acid production under a non-sterile condition was also investigated.
Lactic acid is mostly produced by lactic acid bacteria
via fermentation of refined sugar [1]. Substrate cost is the main expense for lactic acid production. Starch
could be substituted for refined sugar to minimize the production cost. Nevertheless, this is still economically
unfavourable because pretreatment and enzymatic hydrolysis of starch is also costly [2]. A significant
reduction in these costs could be achieved by an application of amylolytic lactic acid bacteria (ALAB) for
simultaneous saccharification and fermentation (SSF) of starch to lactic acid [3]. In addition, among various
starches, cassava starch is the most attractive one in terms of availability and inexpensiveness [4]. National
Innovation Agency, Thailand, estimated that bioconversion of cassava root to polylactic acid could add value
to cassava root by 10 times [5]. However, the study on lactic acid production from SSF of cassava starch is
limited. Thus, the present research aimed at selecting an efficient amylolytic lactic acid bacterial strain and
determining some optimum conditions for SSF of cassava starch to lactic acid. The performance of the selected
strain in lactic acid production under a non-sterile condition was also investigated.
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