The soy sauce
koji
was prepared
after growing the mold (
Aspergillus oryzae
Biokku
Co.
¢
s strain) on a mixture of defatted soybean and
wheat flour (45:55), dried to
c
. 8% moisture, and
then distributed to our laboratory. The
Tetragenococcus
halophilus
IAM1676 was precultured in a
320 mL MRS broth (Merck, Darmstadt, Germany,
modified to pH 7.2 and 7% NaCl added) until
OD
660
=
1.0, collected by centrifugation, resuspended
in 10 mL distilled water, and used as a starter