The genetic relationship of Zostera marina was analyzed by AFLP marker. A total of 235
bands were detected from 63 individuals by 6 primer combinations, of which, 160 (68.09%)
loci were polymorphic. The highest Nei’s gene diversity value (H) and Shannon’s information
index value (I) were detected in population Clare (H ¼ 0.072, I ¼ 0.115), while the
lowest were observed in population Jiaonan (H ¼ 0.007, I ¼ 0.011). Genetic identify values
among 6 populations ranging from 0.66 to 0.92 were observed with the corresponding
distance values ranging from 0.076 to 0.340. The results of AMOVA showed 83.29% genetic
variation was found among populations and 16.71% within populations. According to
UPGMA tree, population Tian’ehu was genetically closer to population Jiaonan than population
Lidao, which might be attributable to eelgrass meadow fragments and some
marine gyres/currents. The geographic distances were responsible for the genetic differentiation
among populations from China, Japan, Korea and Ireland. The results of the
present study emphasized the utility of DNA-based markers for conservation in genetically
depauperate species, any future restoration and conservation projects locally used ecosourced
materials for population augmentations.
The genetic relationship of Zostera marina was analyzed by AFLP marker. A total of 235
bands were detected from 63 individuals by 6 primer combinations, of which, 160 (68.09%)
loci were polymorphic. The highest Nei’s gene diversity value (H) and Shannon’s information
index value (I) were detected in population Clare (H ¼ 0.072, I ¼ 0.115), while the
lowest were observed in population Jiaonan (H ¼ 0.007, I ¼ 0.011). Genetic identify values
among 6 populations ranging from 0.66 to 0.92 were observed with the corresponding
distance values ranging from 0.076 to 0.340. The results of AMOVA showed 83.29% genetic
variation was found among populations and 16.71% within populations. According to
UPGMA tree, population Tian’ehu was genetically closer to population Jiaonan than population
Lidao, which might be attributable to eelgrass meadow fragments and some
marine gyres/currents. The geographic distances were responsible for the genetic differentiation
among populations from China, Japan, Korea and Ireland. The results of the
present study emphasized the utility of DNA-based markers for conservation in genetically
depauperate species, any future restoration and conservation projects locally used ecosourced
materials for population augmentations.
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