as an IS and CH3CN (10L). Then, 30L perchloric acid (60%)
was added for deproteinization, and 800L chloroform, 100L nheptane,
and 100L H2O were added for the extraction of fatty
acids. After the solution was centrifuged at 3000×g for 5 min, the
organic layer (400L) was transferred to a tube and evaporated
under reduced pressure without heating for 22 min. Following the
addition of 10mMp-toluenesulfonic acid (PTSA) in MeOH (200L)
to the dried residue and incubation at 62 ◦C for 2 h for methyl
esterification, H2O (200L) and n-hexane (200L) were sequentially
added, mixed for 2min, and centrifuged at 4000×g for 5min.
The hexane layer (150L), which included fatty acid methyl esters
(FAMEs), was obtained. Then, 10L sample solution was subjected
to GC–MS analysis.
as an IS and CH3CN (10L). Then, 30L perchloric acid (60%)was added for deproteinization, and 800L chloroform, 100L nheptane,and 100L H2O were added for the extraction of fattyacids. After the solution was centrifuged at 3000×g for 5 min, theorganic layer (400L) was transferred to a tube and evaporatedunder reduced pressure without heating for 22 min. Following theaddition of 10mMp-toluenesulfonic acid (PTSA) in MeOH (200L)to the dried residue and incubation at 62 ◦C for 2 h for methylesterification, H2O (200L) and n-hexane (200L) were sequentiallyadded, mixed for 2min, and centrifuged at 4000×g for 5min.The hexane layer (150L), which included fatty acid methyl esters(FAMEs), was obtained. Then, 10L sample solution was subjectedto GC–MS analysis.
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