5. Conclusion
The Adams medium favored the Aspergillus sp. GM4 tannase production and is a low cost medium compared to the other tested culture media. Aspergillus sp. GM4 produces tannase in the presence of tannic acid and methyl gallate inducers. Gallic acid as the sole carbon source induced tannase production. This result suggests that tannase production by Aspergillus sp. GM4 can con- tinue in the presence of increasing product in the culture medium and that the end product does not regulate en- zyme production. In addition, the Plackett-Burman and Central Composite Rotatable designs were shown to be effective in reducing the number of components in the medium composition and increased tannase production 2.66-fold.