erformed incubating
The tests were performed incubating microsomal membranes (0.5 mg/mL of protein) for 5, 10 and 15 min at 30 C
with phenol (100 lM) prior to and subsequent photocatalytic
treatment after removing the nano-TiO2 by centrifugation. Then
100 lL of this incubate was added in the 900 lL of reaction mixture
containing GSH (5 mM) and CDNB (1 mM) and GST activity
was monitored spectrophotometrically at 340 nm. Enzyme activities
were calculated after correction for the nonenzymatic reaction.