After derivatization, 20 lL was injected in the HPLC system on an Alltima reversed-phase C18 column (250 4.6 mm ID, 5 lm particle size; Grace, Lokeren, Belgium)
After derivatization, 20 lL was injected in the HPLC system onan Alltima reversed-phase C18 column (250 4.6 mm ID, 5 lmparticle size; Grace, Lokeren, Belgium)