Results
Identification and phylogenetic analysis of representative
isolates
Based on cultural and morphological characteristics,
80 out of 119 actinomycetes isolated were selected for
molecular identification. Their host sources and
accession numbers are shown in Table 1. The 16S rRNA
gene partial sequence (610 bp) analysis indicated that 66
strains shared 98%−100% of 16S rDNA similarities with
Streptomyces spp. The other 14 strains were consisted of
2 Amycolatopsis, 4 Actinomadura, 1 Nocardia, 2 Microtetraspora,
3 Micromonospora, 1 Kribbella, and 1 Lentzea, indicating
a great taxonomical diversity of endophytic actinomycetes
in these plants. Phylogenetic analysis of the strains from
the 13 medicinal plants was investigated and the reustls
are presented in Fig. 1.
Based on 16S rDNA sequences, phylogenetic trees of the
35 strains from Dioscorea bulbifera, Chenopodium album,
and Populus adenopoda were separately constructed and
analyzed in the present study. Other isolates from Podocarpus