North Pacific squids (Todarodes pacificus) (660 ± 10 g per a
whole squid) were captured from the North Pacific ocean in January
2014 by the East China Sea Fisheries Research Institute, Chinese
Academy of Fishery Sciences and frozen immediately.
Frozen samples were first defrosted in a refrigerator at 4 C overnight,
and then thawed with running water. The internal organs,
arms, and tentacles were removed by hands to obtain the mantle.
The mantle muscle was cut into rectangular sheets with size
of 40.0 ± 5.0 mm in length, 40.0 ± 5.0 mm in width, and
3.0 ± 0.5 mm in thickness. The squid fillets were immersed in
30 g/kg sodium chloride solution at 4 C for 14 h. After salting,
the samples were taken out from the brine solution, quickly rinsed
with distilled water (ca. 30 s) to remove the excessive brine, and
then gently blotted with tissue paper to remove excess water