One-tube nested real-time PCRwas designed to have two sequential reactionswith two sets of primers and dual probes for the insertion sequence (IS) 6110 sequence ofM. tuberculosis in a single closed tube
One-tube nested real-time PCRwas designed to have two sequentialreactionswith two sets of primers and dual probes for the insertion sequence (IS) 6110 sequence ofM. tuberculosis ina single closed tube