The protocol as described in Albillos et al. (2008) was followed.
Whole almonds were ground in a mill and defatted with hexane in
50 mL centrifugal tubes (flour/solvent ratio was 1:10 w/v). Tubes
were shaken at 250 rpm at room temperature (c.a. 22 C) for 3 h.
The solvent was decanted and the extraction process repeated
twice. Residual hexane in the slurry was evaporated in a fume
hood overnight. The defatted almond powder was homogenized
using a mortar with a pestle, and stored at 20 C in airtight plastic
bottles.