In this study, bacterial contamination
did not occur, although sterilization was not conducted.
Using flocculating yeast strain, the continuous ethanol fermentation
of thick juice was performed at relative high dilution rate of
0.11 h1. Bacteria without flocculability were washed out at this
high dilution rate. The relative high ethanol concentration of
80 g L1 in R2 inhibited the growth of bacteria. Besides, the undiluted
thick juice could be directly fed into the reactors, preventing
the feedstock from bacteria contamination