For the enumeration of sulphite-reducing clostridia, 1 ml of the corresponding dilution was heated at 80 C for 10 min and then inoculated in tubes containing Sulphite Polymyxin Sulfadiazine(SPS) melted agar (Pronadisa). To maintain anaerobic conditions, an
overlay of sterile paraffin was added before incubating the tubes at 37 C for 24 h. The results were expressed as cfu/g of sausage.