The PVS2 was removed,
liquid MS medium supplemented with 1.2 M sucrose was added,
Desiccation time (h)
0 5 10 15 20 25 30
Water content (%)
0
10
20
30
40
50
60
Air-dry
Silic a gel
Fig. 1. Fluctuation in Bletilla formosana seed water content during drying with silica
gel.
held for 10 min, removed, and the process was repeated. The liquid
MS medium (0.5 mL) containing 0.4 M sucrose was then added and
held for 10 min before removal and the process was repeated again.
One milligram of the liquid MS medium containing 0.4 M sucrose
was added next and held for 10 min, and then removed. Finally, a
liquid MS medium containing 0.02% Phytagel and 58 mM sucrose
was added and held for 3 d at 25 ◦C in the dark.