Like alcohol inactivation experiment, increasing treatment time
up to 10 min did not show higher killing of germinated spores
with 200 ppm Quats (data not shown). Although ethanol and
Quats are classified as sporostatic compounds that lack abilities to
kill bacterial spores even at longer exposure period (Russell,
1990), our current results demonstrated that initiating spore
germination prior to inactivation with these sporostatic agents
could enhance their spore-killing abilities, especially against
spores of C. perfringensFP isolates.