The sensitive dot blot test can be used to detect the Chlamydia trachomatis infection and other sexually transmitted diseases. Dot blot is used to detect Antidiacyltrehalose Antibodies in Tuberculous patients and Typhoid Fever. This test could increase the number of lives saved that are affected with these diseases. Using the dot blot test can be useful for under-developed countries especially. The dot blot is a good positive predictor of these diseases for countries and regions lacking in medical facilities and laboratories. By using this test many lives could be saved and a cure could be found for the types of diseases this test can detect