a suspension of 1 g of chitin in
50 mL of aqueous sodium hydroxide, as deacetylation reagent (50%
by weight) was mixed at fixed temperature (90e100 C) under
constant stirring. After 3e5 h, the solid was filtered, washed with
water and 80% (v/v) alcohol until the filtrate was neutral. Then it
was oven-dried at 80 C overnight.