2.3. Effects of extract injection and extract immersion
on the resistance of L. vannamei to V. alginolyticus
For the injection test, white shrimp L. vannamei were injected
individually into the ventral sinus of the cephalothorax with the
hot-water extract solutions of S. platensis at 3, 5 and 10 mg ml1
solution at a rate of 20 ml per 10 g weight to reach doses of 6,10, and
20 mg g1, respectively. Shrimp injected with saline (20 ml) and
shrimp with no injection of hot-water extract respectively served
as the saline and control groups.